Just found out some scientists like tattoos.
Our contribution:
1) hkl (on the appropriate knuckles)
2) Bragg’s Law (arm)
3) Diffraction pattern (full back)
Send in pictures of your crystallography tattoos for bonus points.
1) 32 percent on an exam can be a great score
2) 4 mountain dews do not equal a full night of sleep
3) The phrase “if you put it on a slide, you have to know it.”
4) Learn a whole lot about very little
5) Personal hygiene is not universal
6) Snacks offered before a guest lecture do not count as a meal
7) Don’t end a first date with ‘I love you.’
—
What lessons did you or are you learning?
1) Bio-Rad thermal cycler: The PCR song
2) Eppendorf pipetting: Boy Band
Enjoy the weekend!
In true end of the year originality here are 10 favorites from 2009:
1) Best Online Introductions to Crystallography
2) Scientific Research in 10 Simple Rules
3) Free Crystallographic Space Group Diagrams and Symbols
4) Ultimate List of Protein Crystallization Resources
5) Scientific Presentation Resources
6) 17 Structural Journals with RSS
7) Ultimate List on Cryocrystallography & Radiation Damage
Covering your Tracks
9) 10 Ways to Comfort a Crystallographer
10) Top 5 Lies of Principle Investigators
Dear Protein Data Bank,
It’s not you, it’s me.
We’ve been inseparable for what seems like forever, we have been through a lot. Unfortunately, I don’t think that our relationship is going to work out.
I’ve done my best to be patient and even offered suggestions on how we could make things better. I know that you have been improving and even updated your site. I just feel that I need to be better connected to other resources.
Maybe I’m giving up on you too soon.
I’ll miss you,
Sean
P.S. I thought you should know that I’ve been seeing PDBsum lately.
BenchflyTM has videos of protocols, tips and teachings some of which are quite relevant to macromolecular crystallography. The video quality is excellent. I will look forward to watching their future productions even though the buzz intro gives me the creeps.
How to Calibrate Pipettors
Timesaving miniprep tips
Nucleic acid absorbance to concentration
How to remove static from a scale (not for kids)
How to perform colony PCR
Pouring and running an agarose gel
Tip for loading many gel samples quickly
Molarity Tip
Quick answer: hell no.
Here is the equation:
Did that help you?
Did you feel that light bulb go on, like YES! Now I know if this structure is good
Thank goodness they mentioned that equation in 6 point font
Yeah, I get that Rfree came out only 17 years ago and some people still haven’t figured out how to use a seat belt on a plane, but seriously, why is this going on?
In heaven:
Biologists pick the proteins
Chemists evaluate the structures
Physicists do the math
Programmers write code
In hell:
Biologists do the math
Chemists write code
Physicists pick the proteins
Programmers evaluate the structures
Have you ever wanted to go back in time to finish your dissertation research? Now you can!
Through your students!
Who else is going to justify those years of research? You have nothing to lose!
If they fail, it will prove that you tried everything possible!
If they succeed, it will prove that all those hunches you had but never acted on, were correct!