1) Open the desired coordinate files in Coot (click here if you need some help)
cns_generate(You know you have been looking at structures too long when they start to look like faces.)

2) Under Calculate you have two methods of superimposing:
SSM Superpose (we will go with this option in this example: Calculate -> SSM Superpose…) or LSQ Superpose
ssm_align_coot Note: SSM Superpose stands for secondary-structure matching and if you need to do it outside of Coot there is a server.

3) Select which PDB you would like to move and apply:
superimpose_coot
The structures should now be superimposed:
superpose