P212121

10 questions to consider before setting up protein crystallization trials:

1) How pure is your protein sample?

2) What chemicals are present in the protein buffer?

3) Is the protein modified such as phosphorylated, glycosylated or methylated?

4) What temperature and pH is the protein stable?

5) What concentration causes the protein to precipitate out of solution?

6) Does the protein have any known substrates, metals, inhibitors or ligands?

7) Have related proteins been crystallized?

Is the protein sensitive to proteolysis?

9) Does the protein have cysteines?

10) How has the protein been stored?

Feel free to add any questions that you consider in the comments.