Coot: How to Simultaneously Mutate Multiple Residues
Dec 21, 2009
If you need to mutate multiple residues simultaneously there is a great option to use in Coot instead of mutating them individually.
Calculate -> Mutate Residue Range…
1) Make sure you are mutating the correct PDB file
2) Select Chain
3) Enter the residues range by number that you would like mutated
4) Enter desired sequence
5) Option for to autofit the mutated residues (you need to have a map for this)
What are some of the possible uses?
1) Create a poly-alanine chain
2) Mutate a structure that varies between species
3) Mutate hyper variable regions
Note: You cannot add residues with this option.
Posted by Sean |
Categories: Crystallographic Data Processing, Crystallographic Data Refinement | Tagged: Coot, Crystallographic Data Processing, Crystallographic Data Refinement, Learning |
