How to get started with Coot
The very basics of using Coot for those that have not used this program. I am a big fan of Coot and think if you give it a try then maybe you will be too. This post will cover three basics: 1) opening a PDB file, 2) loading maps, and 3) how to move about using your mouse.
1) Opening a PDB file with Coot
Find your desired PDB file (coordinates) and select it
Tip: Using the Filter option can save a lot of time
2) Loading a 2Fo-Fc map
Simply hit ‘Ok’ and you did it!
Here is how to load a Fo-Fc map:
Select (its below the default option) and open with the ‘DEL’ for the phases and amplitudes
3) Moving the macromolecule with your mouse
Left click (hold) allows you to rotate the coordinates
Right click (hold) allows you to zoom in (move toward you or right) and out (move away or left)
Center click allows you to center (focus) on an atom
