Comments on: Easy to Replicate http://www.P212121.com/2009/09/13/easy-to-replicate/ Protein Crystallography there is an Easier Way Mon, 06 Sep 2010 23:18:47 -0400 http://wordpress.org/?v=2.8.5 hourly 1 By: Sili http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1498 Sili Sat, 16 Jan 2010 20:03:36 +0000 http://www.P212121.com/?p=4410#comment-1498 I recall hearing about solutions that grew neutronsource size crystals in the boot of a car - on the way back from a synchrotron. I recall hearing about solutions that grew neutronsource size crystals in the boot of a car – on the way back from a synchrotron.

]]> By: Sean http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1154 Sean Fri, 25 Sep 2009 03:46:14 +0000 http://www.P212121.com/?p=4410#comment-1154 Hello Cory, Thanks for wonderful reply and welcome to P212121! Also thanks again for taking the time to let me use Plug Maker at the ACA. This is the best expansion that I have had so far and have given it a pretty good effort (over 1200 conditions set up so far). So Samia, don't get too jealous. Hello Cory,

Thanks for wonderful reply and welcome to P212121!

Also thanks again for taking the time to let me use Plug Maker at the ACA. This is the best expansion that I have had so far and have given it a pretty good effort (over 1200 conditions set up so far).

So Samia, don’t get too jealous.

]]> By: Cory http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1148 Cory Tue, 22 Sep 2009 15:28:11 +0000 http://www.P212121.com/?p=4410#comment-1148 Hey Sean, I have some ideas on reproducing the crystals. 1. Determining the concentration of the precipitant is straight forward as long as you know the starting and ending conditions. In fact, since we did a slow smooth gradient, an interpolation of the precipitant concentration is very accurate. 2. For experiment style, batch-under-oil style crystallization would be the most similar to the original experiment. 3. Don't forget that you can harvest those crystals!!! The CrystalCard has a Peel-Apart backing so you can open it up and pull the crystals out. Hey Sean,

I have some ideas on reproducing the crystals.

1. Determining the concentration of the precipitant is straight forward as long as you know the starting and ending conditions. In fact, since we did a slow smooth gradient, an interpolation of the precipitant concentration is very accurate.

2. For experiment style, batch-under-oil style crystallization would be the most similar to the original experiment.

3. Don’t forget that you can harvest those crystals!!! The CrystalCard has a Peel-Apart backing so you can open it up and pull the crystals out.

]]> By: Sean http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1143 Sean Fri, 18 Sep 2009 20:41:45 +0000 http://www.P212121.com/?p=4410#comment-1143 Samia! Great to hear from you! Haha, my first reaction was about the same :) I have some ideas about reproducing the condition - thinking sitting drop, but need to think about it some more. One issue is how to determine the exact condition that produced the crystals (ie. what is the concentration of the precipitate?). I can estimate, but as far as I can tell there is not a way to determine it exactly so may need to do some optimization. Peter, do you have a protocol in place for reproducing crystals from the crystal card? Samia! Great to hear from you!

Haha, my first reaction was about the same :)

I have some ideas about reproducing the condition – thinking sitting drop, but need to think about it some more.

One issue is how to determine the exact condition that produced the crystals (ie. what is the concentration of the precipitate?). I can estimate, but as far as I can tell there is not a way to determine it exactly so may need to do some optimization.

Peter, do you have a protocol in place for reproducing crystals from the crystal card?

]]> By: Samia http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1136 Samia Thu, 17 Sep 2009 00:40:38 +0000 http://www.P212121.com/?p=4410#comment-1136 My first thoughts upon reading this were: 1) WHOA 2) How the heck are you going to reproduce those conditions??? Amazing. Congrats on the little beauties! My first thoughts upon reading this were:

1) WHOA
2) How the heck are you going to reproduce those conditions???

Amazing. Congrats on the little beauties!

]]> By: Sean http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1121 Sean Tue, 15 Sep 2009 02:05:39 +0000 http://www.P212121.com/?p=4410#comment-1121 Size: about 25 by 30 micro millimeters, hard to get the third dimension due to the plastic Loop: have yet to try, but yeah I can loop them ;) Reproduce: working on it, this just happened :) Size: about 25 by 30 micro millimeters, hard to get the third dimension due to the plastic
Loop: have yet to try, but yeah I can loop them ;)
Reproduce: working on it, this just happened :)

]]> By: enrico http://www.P212121.com/2009/09/13/easy-to-replicate/comment-page-1/#comment-1113 enrico Mon, 14 Sep 2009 05:13:50 +0000 http://www.P212121.com/?p=4410#comment-1113 Wow, I wonder if and when you'll try to reproduce those crystals! Great story by the way.. How big are those crystals? Can you loop them? Did you try to reproduce them with a different setting (ie hanging drops)? Cheers, E. Wow, I wonder if and when you’ll try to reproduce those crystals! Great story by the way..
How big are those crystals? Can you loop them? Did you try to reproduce them with a different setting (ie hanging drops)?
Cheers,
E.

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