P212121

I have come across quite a few papers and books about protein crystallization. Unfortunately, I have not yet found a resource that answers the more practical questions about crystallization:

Typical Questions:

1) What should I read to understand the basic theory of protein crystallization?

2) How much protein do I need for crystallization?
(Note: Peter did a good post on this topic: Risky business or, how much protein sample do you need?)

3) How much will it cost to attain a protein crystal?

4) What is the ideal buffer for my protein?

5) Which method should I use for crystallization?

6) What concentration should I use when setting up my crystallization trails?

7) What size should my drops be during expansion set ups?

When should I expect to see crystals?

9) How can I tell that I have salt crystals and not protein crystals?

10) What screen(s) should you use and in what order?

Have I left any questions out?

Please feel free to add more questions below in the comments section or send me an email: sean@p212121.com

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