Salt Crystal Predictor and Quick Lookup

May 28, 2009

I have been working with a protein that is quite soluble in sodium potassium phosphate (yes, I should find another buffer). I set up crystal trays using screens from each of the major of screen manufactures: Hampton Research, Qaigen, Microlytic and Emerald Biosystems.

I came across a number of conditions containing magnesium chloride so was worried about the formation of magnesium phosphate crystals instead of protein crystals. I then decided to search for a tool that would allow me to quickly identify what conditions have the ‘possibility’ of forming salt crystals. I came across a site called the crystal screen wizard with a tool called the Salt Crystal Predictor. The tool is very easy to use:

1) select a screen (if it is not listed then you can upload your own)
2) write out the protein buffer condition is present while screening

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In addition, if you do not like flipping through pages of what conditions are in which well for a particular screen, you may also find this tool helpful.

Quick Lookup

1) select a screen 2) condition number –> outputs the condition

quicklookup Salt Crystal Predictor and Quick Lookup

I would like if the tool did not send the user to a separate page after clicking submit, but is still a time saver.

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